Edvotek DNA Fingerprinting by PCR Amplification Experiment Kit

Forensic DNA fingerprinting has become a universally accepted crime-fighting tool. Recent advances use the polymerase chain reaction (PCR) to amplify human DNA obtained from crime scenes. This experiment, based on a crime scene scenario, has an inquiry-based component.

• Learn about DNA fingerprinting while solving a crime!
• Introduce the biotechnologies of restriction enzyme digestion, PCR, and electrophoresis
• Perform agarose gel electrophoresis to separate differently-sized DNA molecules
• Load, run, and analyze Ready-to-Load™ PCR reactions obtained from suspects and the crime scene
• Group size: for 8 gels
• Time required: complete in 45 minutes
• Kit includes: instructions, ready-to-load QuickStrip™ DNA samples, UltraSpec-Agarose™, electrophoresis buffer (50X), practice gel loading solution, FlashBlue™ DNA stain
• You will need: horizontal gel electrophoresis apparatus, D.C. power supply, automatic micropipettes with tips, balance, microwave/hot plate/burner, pipette pump, 250mL flasks/beakers, hot gloves, safety goggles, disposable laboratory gloves, small plastic trays/large weight boats (for gel destaining), DNA visualisation system (white light), distilled/deionised water